Optical Aligment

To do next before 3D imaging:

  1. Laser light beam check and collimation (Collimating lens)
  2. Laser light beam alignments (Collimating lens and Mirror 1, 2 and 3)
  3. Cylindrical lens installation (Cylindrical lens 1 or 2 with x-stage)
  4. Actuators and camera setup (Sample stage and detection optics)
  5. Fluorescence signal check (Sample stage and detection optics)
  6. Follow-up alignments (Cylindrical lens and detection optics; if required)

PDF